Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
Reporter expression is observed in all cells of the eye but expression levels vary. Expression levels are reduced just posterior to the morphogenetic furrow and increase again at around columns 2 to 3. Anterior to the morphogenetic furrow, expression levels are higher in the ventral part of the disc compared to the dorsal part and especially high at the dorsal-ventral equator. Posterior to the furrow, expression levels remain constant in undifferentiatled cells but are dynamic in differentiating cells. Levels are high in R3, R4, and R8 nuclei when first identified and low in R2 and R5 cells. Levels are high in R1 and R6 nuclei when first identified around column 6 but decrease from column 8 onwards. Expression levels are high in R7 and cone cell precursors from their appearance in columns 8 and 10, respectively. Levels remain high in R3/R4 and R7 photoreceptor cells and in cone cells but drop in R8 photoreceptor cells.
ms(3)03970
l(3)03970
Location 3L:732873-732874 determined by FlyBase alignment of dbGSS accession AQ026429 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).