FlyBase Insertion Report: Dmel\P{PZ}Rb97D[1]

General Information

Symbol

Dmel\P{PZ}Rb97D¹

Species

D. melanogaster

Name

FlyBase ID

FBti0003551

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

ms(3)03445

Affected gene(s)

Ecol\lacZ, Rb97D

Viability / fertility

Associated allele(s)

Ecol\lacZ^Rb97D-1, Rb97D¹

Stock availability

None publicly available

Genomic Location

Chromosomal Location

3R ( 97D3 )

Sequence Location

3R:26,875,695..26,875,695 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:26,875,695..26,875,695 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

97D3 (inferred by FlyBase from sequence location)

Cytological location (reported)

97D1-97D15 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026425

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Zhang et al., 2000, Zhang and Stankiewicz, 1998, Castrillon et al., 1993, Karsch-Mizrachi and Haynes, 1993)

Rb97D

(Ustaoglu et al., 2019, Sofola et al., 2007, Castrillon et al., 1993)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^Rb97D-1

Phenotypic Class Phenotype Manifest in Detailed Description

Rb97D¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

spermatogenesis & adult stage

testis

(Karsch-Mizrachi and Haynes, 1993)

spermatid

(Karsch-Mizrachi and Haynes, 1993)

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage & spermatogenesis

testis

(Karsch-Mizrachi and Haynes, 1993)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

gonad

(Meister and Braun, 1995.10)

spermatogenesis & adult stage

spermatocyte

(Karsch-Mizrachi and Haynes, 1993)

Additional Information

Statement

Reference

Strong staining is seen in the larval and adult testis. In the adult, staining is seen in spermatocytes and spermatids, but not in the stem cells at the apex of the testis or the terminal epithelium at the base.

(Karsch-Mizrachi and Haynes, 1993)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

ms(3)03445

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

Location 3R:22701417-22701418 confirmed by FlyBase alignment of dbGSS accession AQ026425 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1782

(Meister and Braun, 1995.10)

P{PZ}Hrb97D¹

(FlyBase, 1992-)

P{PZ}Rb97D⁰³⁴⁴⁵

(FlyBase, 1992-)

P{PZ}Rb97D¹

(Ustaoglu et al., 2019, FlyBase, 2005, Castrillon et al., 1993, FlyBase, 1992-)

P{PZ}ms(3)03445⁰³⁴⁴⁵

(FlyBase, 1992-)

P{ry^+t7.2=PZ}Rb97D¹

(FlyBase, 1992-)

rb97D¹

(Zhang et al., 2000)

Name Synonyms

Secondary FlyBase IDs

FBti0000204

References (17)

Report Sections

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