FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{PZ}E2f1rM729
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General Information
Symbol
Dmel\P{PZ}E2f1rM729
Species
D. melanogaster
Name
FlyBase ID
FBti0003853
Feature type
transposable_element_insertion_site
Also Known As
E2F-lacZ, e2f1-lacZ
Description
Inserted Element
P{PZ}
LINE ID

l(3)rM729

Affected gene(s)
E2f1, Ecol\lacZ
Viability / fertility
Associated allele(s)
E2f1rM729, Ecol\lacZE2f1-rM729
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
3R ( 93E9 )
Sequence Location
3R:21,626,545..21,626,545 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{PZ}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
3R:21,626,545..21,626,545 [-]
(Duronio et al., 1995)
Orientation
Cytological location (computed by FlyBase)
93E9 (inferred by FlyBase from sequence location)
Cytological location (reported)
93E8-93E9 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
P{PZ}
Size (Kb)

14.545

Component Allele(s)
l(3)87Df7.2
ry+t7.2
Molecular map
Affected Gene(s)
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
presumptive embryonic/larval central nervous system
(Duronio et al., 1995)
embryonic stage 10 -- 17
organism | ubiquitous
(Duronio et al., 1995)
Additional Information
Statement
Reference

Enhancer trap expression is ubiquitous and nearly uniform after germ band extension. Especially high transcript levels are seen in proliferating CNS cells.

(Duronio et al., 1995)
 
Marker for
Reflects expression of
E2f1
(Duronio et al., 1995)
Reporter construct used in assay
Data on Genetic Line
Line ID

l(3)rM729

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

P{PZ}E2frM729 is inserted 48 nucleotides upstream of the E2f translation start and in the same orientation as E2f.

(Duronio et al., 1995)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
E2F-lacZ
(Kugler and Nagel, 2007, Delanoue et al., 2004)
E2Frm729
(Delanoue et al., 2004)
P{PZ}E2f1rM729
P{PZ}E2frM729
(Duronio et al., 1995, FlyBase, 1992-)
dE2F729
(Hirose et al., 2001)
e2f1-lacZ
(Li et al., 2024, Bar-Cohen et al., 2023)
Name Synonyms
Secondary FlyBase IDs
    References (34)