Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
In embryo, expressed in the CNS midline. In embryo, expression in hindgut, midgut, pharynx and ectodermal stripes described as "basic" expression pattern; observed in many lines (FBrf0080145).
l(2)k06921
The P{lacW}Sk06921 insertion maps 1060bp upstream of the S AUG codon on the - strand and 275bp upstream of the ast AUG codon on the + strand and may therefore affect both genes.
The P{lacW}Sk06921 insertion site was reported as approximately 1060bp upstream of the S AUG codon. The orientation of the insertion was not reported.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).