FlyBase Insertion Report: Dmel\P{PZ}bnl[06916]

General Information

Symbol

Dmel\P{PZ}bnl⁰⁶⁹¹⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0004144

Feature type

transposable_element_insertion_site

Also Known As

bnl-lacZ

Description

Inserted Element

P{PZ}

LINE ID

l(3)06916

Affected gene(s)

bnl, Ecol\lacZ

Viability / fertility

Associated allele(s)

bnl⁰⁶⁹¹⁶, Ecol\lacZ^bnl-06916

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 92B3 )

Sequence Location

3R:19,836,868..19,836,868 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3R:19,836,868..19,836,868 [+]

Orientation

(Sutherland et al., 1996)

Cytological location (computed by FlyBase)

92B3 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Mapped to the region 3R:15659822..15663440 (SalI-HindIII restriction fragment); r5 coordinates. The insertion is in a negative orientation with respect to bnl gene transcription.

(Sutherland et al., 1996)

Sequence Data

Flanking sequence

G00595

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

bnl

(Yan and Lin, 2007, Sutherland et al., 1996, BDGP Project Members, 1994-1999)

Ecol\lacZ

(Chatterjee et al., 2011, Srahna et al., 2006, Ahmad and Baker, 2002, Sato and Kornberg, 2002, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

bnl⁰⁶⁹¹⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^bnl-06916

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

In wandering third instar larva, no detectable β-gal staining.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

bnl

(Yan and Lin, 2007)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)06916

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11704

ry⁵⁰⁶ P{PZ}bnl⁰⁶⁹¹⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

The insertion site maps to a SalI-HindIII restriction site. The insertion is in a negative orientation with respect to bnl gene transcription.

(Sutherland et al., 1996)

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

P1704

(Meister and Braun, 1995.10)

P{PZ}bnl⁰⁶⁹¹⁶

(Sutherland et al., 1996, FlyBase, 1992-)

P{PZ}l(3)06916⁰⁶⁹¹⁶

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}l(3)neo52⁰⁶⁹¹⁶

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)neo52⁰⁶⁹¹⁶

(FlyBase, 1992-)

bnl-lacZ

(Du et al., 2017, Grifoni et al., 2015, Chatterjee et al., 2011, Yan and Lin, 2007, Srahna et al., 2006, Ahmad and Baker, 2002)

bnl⁰⁶⁹¹⁶

(Chatterjee et al., 2011)

Name Synonyms

Secondary FlyBase IDs

FBti0000671
FBti0005571

References (18)

Report Sections

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