FlyBase Insertion Report: Dmel\P{PZ}retn[05096]

General Information

Symbol

Dmel\P{PZ}retn⁰⁵⁰⁹⁶

Species

D. melanogaster

Name

FlyBase ID

FBti0004265

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)05096

Affected gene(s)

AP-2α, Ecol\lacZ, retn

Viability / fertility

Associated allele(s)

Ecol\lacZ^retn-05096, retn⁰⁵⁰⁹⁶

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 37E5 )

Sequence Location

2L:19,520,143..19,520,143

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

(Treisman and Rubin, 1996)

Sequence Location

2L:19,520,143..19,520,143

(Shandala et al., 1999)

Orientation

Cytological location (computed by FlyBase)

37E5 (inferred by FlyBase from sequence location)

Cytological location (reported)

59F1-59F4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

The insertion site was reported as 439 bp upstream of the retn transcription start site and 32 bp downstream of the P{PZ}retn⁰²⁵³⁵ insertion site.

(Shandala et al., 1999)

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

AP-2α

(Treisman and Rubin, 1996)

Ecol\lacZ

(Shandala et al., 1999, Treisman and Rubin, 1996)

retn

(Shandala et al., 1999, Spradling et al., 1999, Treisman and Rubin, 1996, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^retn-05096

Phenotypic Class Phenotype Manifest in Detailed Description

retn⁰⁵⁰⁹⁶

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

eye disc

(Treisman and Rubin, 1996)

Additional Information

Statement

Reference

For eye disc, described as "expressed late."

(Treisman and Rubin, 1996)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)05096

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

The orientation of the insertion was not reported.

(Shandala et al., 1999)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

2-5096

(Treisman and Rubin, 1996)

P{PZ}dri⁰⁵⁰⁹⁶

(FlyBase, 1992-)

P{PZ}l(2)02535⁰⁵⁰⁹⁶

(Treisman and Rubin, 1996, FlyBase, 1992-)

P{PZ}retn⁰⁵⁰⁹⁶

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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