FlyBase Insertion Report: Dmel\P{PZ}wb[09437]

General Information

Symbol

Dmel\P{PZ}wb⁰⁹⁴³⁷

Species

D. melanogaster

Name

FlyBase ID

FBti0005158

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

Uses

lacZ reporter

Affected gene(s)

Ecol\lacZ, wb

Viability / fertility

Associated allele(s)

Ecol\lacZ^wb-09437, wb⁰⁹⁴³⁷

Stock availability

1 publicly available

LINE ID

l(2)09437

Genomic Location

Chromosomal Location

2L ( 35A3-35A4 )

Sequence Location

2L:14,288,960..14,319,552 [+]

Target / Docking site

Genomic Maps

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Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

35A3-35A4 (inferred by FlyBase from sequence location)

Cytological location (reported)

35A1-35A2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00417

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Location-dependent role

lacZ enhancer trap

(Mlodzik and Hiromi, 1992)

Size (Kb)

14.545

Associated alleles

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

(Thuveson et al., 2019, Han et al., 2012, BDGP Project Members, 1994-1999)

Alleles and Phenotypes

Allele

Ecol\lacZ^wb-09437

Reference(s)

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Allele

wb⁰⁹⁴³⁷

Reference(s)

(Thuveson et al., 2019, Han et al., 2012, Brown and Freeman, 2003, Ashburner et al., 1999, Ashburner et al., 1999, Ashburner et al., 1999.10.12, Martin et al., 1999, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Data

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

epidermis | restricted