FlyBase Insertion Report: Dmel\P{PZ}lilli[00632]

General Information

Symbol

Dmel\P{PZ}lilli⁰⁰⁶³²

Species

D. melanogaster

Name

FlyBase ID

FBti0005171

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)00632

Affected gene(s)

Ecol\lacZ, lilli

Viability / fertility

Associated allele(s)

Ecol\lacZ^lilli-00632, lilli⁰⁰⁶³²

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 23C1 )

Sequence Location

2L:2,888,923..2,888,923 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:2,888,923..2,888,923 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

23C1 (inferred by FlyBase from sequence location)

Cytological location (reported)

23C1-23C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ025583

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Distefano et al., 2012, Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

lilli

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^lilli-00632

Phenotypic Class Phenotype Manifest in Detailed Description

lilli⁰⁰⁶³²

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

type II neuroblast

(Liu et al., 2017)

third instar larval stage

retina | ubiquitous

(Distefano et al., 2012)

wandering third instar larval stage

embryonic/larval midgut

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

adult stage

adult brain

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

The reporter accumulates specifically in neuroblasts and is quickly downregulated in differentiating progeny cells.

(Liu et al., 2017)

The lilli reporter is expressed ubiquitously in the third instar retina.

(Distefano et al., 2012)

In adult, expression throughout brain.

(Vosshall, 1998.8.10)

Marker for

Reflects expression of

lilli

(Liu et al., 2017, Distefano et al., 2012)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)00632

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

10944

P{PZ}lilli⁰⁰⁶³² cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2L:2888923-2888924 confirmed by FlyBase alignment of dbGSS accession AQ025583 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P{PZ}SY2-1⁰⁰⁶³²

(FlyBase, 1992-)

P{PZ}l(2)00632⁰⁰⁶³²

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}lilli⁰⁰⁶³²

(FlyBase, 2005, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)00632⁰⁰⁶³²

(FlyBase, 1992-)

dAFF-lacZ

(Liu et al., 2017)

lilli⁰⁰⁶³²

(Distefano et al., 2012)

Name Synonyms

Secondary FlyBase IDs

FBti0000248

References (18)

Report Sections

Open Close