FlyBase Insertion Report: Dmel\P{PZ}02321

General Information

Symbol

Dmel\P{PZ}02321

Species

D. melanogaster

Name

FlyBase ID

FBti0005213

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)02321

Affected gene(s)

Ecol\lacZ, oaf

Viability / fertility

viable

Associated allele(s)

Ecol\lacZ⁰²³²¹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2L ( 22F4 )

Sequence Location

2L:2,494,124..2,494,124 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2L:2,494,124..2,494,124 [-]

(Gene Disruption Project members, 2015.10.8)

Orientation

(Gene Disruption Project members, 2015.10.8)

Cytological location (computed by FlyBase)

22F4 (inferred by FlyBase from sequence location)

Cytological location (reported)

22F4_r6 (reported as inferred from sequence location)

(Gene Disruption Project members, 2015.10.8)

22F3--22F4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

22F3-22F4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

invader2

(Gene Disruption Project members, 2015.10.8)

Comments concerning location

Inserted in a copy of invader2, mapped by unique segment at 5prime end of 5prime flank.

(Gene Disruption Project members, 2015.10.8)

Sequence Data

Flanking sequence

KS308164

(Gene Disruption Project members, 2015.10.8)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Siegmund and Korge, 2001, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

oaf

(Gene Disruption Project members, 2015.10.8)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰²³²¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

larval insulin secreting cell of pars intercerebralis

(Siegmund and Korge, 2001)

wandering third instar larval stage

larval brain

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, bilateral pattern of expression in the central brain.

(Vosshall, 1998.8.10)

In wandering third instar larva, low levels of β-gal expression in the larval midgut and brain.

(Meister and Braun, 1995.10)

Marker for

larval insulin secreting cell of pars intercerebralis

(Siegmund and Korge, 2001)

Reflects expression of

l(2)02321

(Meister and Braun, 1995.10)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)02321

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11183

P{PZ}02321 cn¹, l(2)02321⁰²³²¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Insertion separable from lethal originally isolated on the same chromosome.

(BDGP Project Members, 1994-1999)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1183

(Siegmund and Korge, 2001, Meister and Braun, 1995.10)

P{PZ}02321

(FlyBase, 1992-)

P{PZ}l(2)02321⁰²³²¹

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)02321⁰²³²¹

(FlyBase, 1992-)

l(2)02321

(Gene Disruption Project members, 2015.10.8)

Name Synonyms

Secondary FlyBase IDs

FBti0000292

References (10)

Report Sections

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