tnd, l(2)ND1, l(2)22Fb
Please see the JBrowse view of Dmel\oaf for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Stop-codon suppression (UGA) postulated; FBrf0079893.
Gene model reviewed during 5.47
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
3.3, 2.4-3.0, 1.8-2.0 (northern blot)
487, 322 (aa); 54, 37 (kD predicted)
The oaf gene contains an open reading frame
which encodes a protein of 322aa followed by a UGA stop codon immediately
followed by another ORF with the potential to encode another 155 amino
acids.
The oaf gene contains an open reading frame
which encodes a protein of 322aa followed by a UGA stop codon immediately
followed by another ORF with the potential to encode another 155 amino
acids. A 487aa oaf protein is potentially made if the UGA codon is
supressed by incorporation of selenocysteine at this codon.
the 155 additional amino acids which would be produced if there was a readthrough at the stop codon for oaf+P332
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\oaf using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as procephalic ectoderm anlage
Comment: reported as dorsal epidermis anlage
The 3.3kb oaf transcript is expressed throughout development. It is present at high levels in early embryos, declines through embryonic and larval stages and increases again in pupae and adults.
The group 2 oaf transcripts are abundant in early embryos and adult females.
The group 3 oaf transcripts are abundant in 0-8hr embryos and become rare in older embryos and larvae. Levels increase in late third instar larvae, pupae and adults.
JBrowse - Visual display of RNA-Seq signals
View Dmel\oaf in JBrowse
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dpp enhancers ignore the nearer Slh and oaf promoters while activating transposon promoters (P{PZ} insertion into oaf, oafE-32). This use of only some promoters in the region strongly supports the notion that promoter specificity is the overriding feature establishing the regulatory autonomy of these genes.
Phenotypic analysis suggests that oaf is necessary for proper neuronal development and hatching.
Phenotypic analysis of mutations suggest oaf may be required for proper neural development.
Complementation analysis demonstrates oaf is equivalent to l(2)ND1.
The gene has been named 'out at first' based on the mutant phenotype, death during the first larval instar.
