FlyBase Insertion Report: Dmel\P{PZ}ValRS[03531]

General Information

Symbol

Dmel\P{PZ}ValRS⁰³⁵³¹

Species

D. melanogaster

Name

FlyBase ID

FBti0005240

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)03531

Affected gene(s)

Ecol\lacZ, Kdm4B, ValRS

Viability / fertility

Associated allele(s)

Ecol\lacZ^ValRS-03531, Kdm4B⁰³⁵³¹, ValRS⁰³⁵³¹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 49F7 )

Sequence Location

2R:13,198,020..13,198,020 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:13,198,020..13,198,020 [+]

Orientation

Cytological location (computed by FlyBase)

49F7 (inferred by FlyBase from sequence location)

Cytological location (reported)

49F7-49F8 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00583

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Kdm4B

(Palomera-Sanchez et al., 2010, FlyBase, 1996-)

ValRS

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^ValRS-03531

Phenotypic Class Phenotype Manifest in Detailed Description

Kdm4B⁰³⁵³¹

Phenotypic Class Phenotype Manifest in Detailed Description

ValRS⁰³⁵³¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

gastric caecum

(Jackle and Janning, 1998.8.10)

organism | segmentally repeated

(Jackle and Janning, 1998.8.10)

embryonic/larval midgut

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval central nervous system | restricted

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval midgut

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In mid-stage embryos, segmentally repeated expression may correspond to neuroblasts.

(Jackle and Janning, 1998.8.10)

In third instar larva, highest levels of expression in Malpighian tubules.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)03531

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11336

cn¹ P{PZ}ValRS⁰³⁵³¹ Kdm4B⁰³⁵³¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

P1336

(Meister and Braun, 1995.10)

P{PZ}Aats-val⁰³⁵³¹

(FlyBase, 1992-)

P{PZ}ValRS⁰³⁵³¹

P{PZ}l(2)03531⁰³⁵³¹

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)03531⁰³⁵³¹

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000321

References (11)

Report Sections

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