FlyBase Insertion Report: Dmel\P{PZ}Cdk4[05428]

General Information

Symbol

Dmel\P{PZ}Cdk4⁰⁵⁴²⁸

Species

D. melanogaster

Name

FlyBase ID

FBti0005292

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)05428

Affected gene(s)

Cdk4, Ecol\lacZ

Viability / fertility

Associated allele(s)

Cdk4⁰⁵⁴²⁸, Ecol\lacZ^Cdk4-05428

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 53C9 )

Sequence Location

2R:16,583,891..16,583,891 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:16,583,891..16,583,891 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

53C9 (inferred by FlyBase from sequence location)

Cytological location (reported)

53C9-53C10 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Genomic location previously reported as 2R:12471387..12471395 .

Sequence Data

Flanking sequence

AQ025637

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Cdk4

(BDGP Project Members, 1994-1999)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Cdk4⁰⁵⁴²⁸

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^Cdk4-05428

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

embryonic/larval anterior midgut

(Jackle and Janning, 1998.8.10)

gastric caecum

(Jackle and Janning, 1998.8.10)

epidermis | segmentally repeated

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

adult stage

proboscis

(Vosshall, 1998.8.10)

adult fat body

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In wandering third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)05428

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11407

cn¹ P{PZ}Cdk4⁰⁵⁴²⁸/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Comments

Location 2R:12098754-12098755 confirmed by FlyBase alignment of dbGSS accession AQ025637 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

insertion associated host repeat

P element insertion is in opposite orientation with respect to Cdk4 gene transcription.

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1407

(Meister and Braun, 1995.10)

P{PZ}Cdk4⁰⁵⁴²⁸

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}l(2)05428⁰⁵⁴²⁸

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)05428⁰⁵⁴²⁸

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000377

References (13)

Report Sections

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