FlyBase Insertion Report: Dmel\P{PZ}06320

General Information

Symbol

Dmel\P{PZ}06320

Species

D. melanogaster

Name

FlyBase ID

FBti0005306

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)06320

Affected gene(s)

Ecol\lacZ

Viability / fertility

Associated allele(s)

Ecol\lacZ⁰⁶³²⁰

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2L ( 30D3-30D4 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

30D3-30D4 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰⁶³²⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In wandering third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)06320

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

P{PZ}06320 is viable in combination with Df(2L)s1402, a deletion removing the P insertion site. Consequently, the lethality of the P insertion chromosome does not map to the insertion site.

(Cook, 2014.9.25)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P1482

(Meister and Braun, 1995.10)

P{PZ}06320

P{PZ}l(2)06320⁰⁶³²⁰

(Cook, 2014.9.25, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)06320⁰⁶³²⁰

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000392

References (10)

Report Sections

Open Close