FlyBase Insertion Report: Dmel\P{PZ}grh[06850]

FB2026_01 , released March 12, 2026

FB2026_01 , released March 12, 2026

Insertion: Dmel\P{PZ}grh⁰⁶⁸⁵⁰

General Information

Symbol

Dmel\P{PZ}grh⁰⁶⁸⁵⁰

Species

D. melanogaster

Name

FlyBase ID

FBti0005317

Feature type

transposable_element_insertion_site

Description

Inserted Element

LINE ID

l(2)06850

Affected gene(s)

Viability / fertility

Associated allele(s)

Ecol\lacZ^grh-06850, grh⁰⁶⁸⁵⁰

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

2R ( 54F1 )

Sequence Location

2R:17,822,221..17,822,221 [+]

Target / Docking site

Genomic Maps

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:17,822,221..17,822,221 [+]

Orientation

Cytological location (computed by FlyBase)

54F1 (inferred by FlyBase from sequence location)

Cytological location (reported)

54F1-54F2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

(FlyBase, 1992-)

Inserted Element

Construct

Size (Kb)

14.545

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

(Jackle and Janning, 1998.8.10, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

(Dominado et al., 2025, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^grh-06850

Phenotypic Class Phenotype Manifest in Detailed Description

grh⁰⁶⁸⁵⁰

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

(Meister and Braun, 1995.10)

Additional Information

Statement

Reference

In mid-stage embryo, expression anterior to the cephalic furrow. In later embryo, expression in PNS uncertain.

(Jackle and Janning, 1998.8.10)

In wandering third instar, weak β-gal staining in the larval brain.

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)06850

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

12325

P{PZ}grh⁰⁶⁸⁵⁰ cn¹/CyO; ry⁵⁰⁶

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P2325

(Meister and Braun, 1995.10)

P{PZ}grh⁰⁶⁸⁵⁰

(FlyBase, 1992-)

P{PZ}l(2)06850⁰⁶⁸⁵⁰

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)06850⁰⁶⁸⁵⁰

(FlyBase, 1992-)

grh⁰⁶⁸⁵⁰-lacz

(Díaz-de-la-Peña et al., 2020)

grhlacZ

(Díaz-de-la-Peña et al., 2020)

Name Synonyms

Secondary FlyBase IDs

FBti0000404

References (13)

Report Sections