FlyBase Insertion Report: Dmel\P{PZ}l(2)10521[10521]

General Information

Symbol

Dmel\P{PZ}l(2)10521¹⁰⁵²¹

Species

D. melanogaster

Name

FlyBase ID

FBti0005352

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(2)10521

Affected gene(s)

Dpit47, Ecol\lacZ, l(2)10521

Viability / fertility

Associated allele(s)

Ecol\lacZ¹⁰⁵²¹, l(2)10521¹⁰⁵²¹

Stock availability

None publicly available

Genomic Location

Chromosomal Location

2R ( 42C3 )

Sequence Location

2R:6,661,934..6,661,934 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

2R:6,661,934..6,661,934 [-]

(Gene Disruption Project members, 2015.10.8)

Orientation

(Gene Disruption Project members, 2015.10.8)

Cytological location (computed by FlyBase)

42C3 (inferred by FlyBase from sequence location)

Cytological location (reported)

42C3_r6 (reported as inferred from sequence location)

(Gene Disruption Project members, 2015.10.8)

42C1-42C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

KS308202

(Gene Disruption Project members, 2015.10.8)

KS308201

(Gene Disruption Project members, 2015.10.8)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Dpit47

(Gene Disruption Project members, 2015.10.8)

Ecol\lacZ

(Jackle and Janning, 1998.8.10, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

l(2)10521

(BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ¹⁰⁵²¹

Phenotypic Class Phenotype Manifest in Detailed Description

l(2)10521¹⁰⁵²¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism | segmentally repeated

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval peripheral nervous system

(Jackle and Janning, 1998.8.10)

embryonic/larval hindgut

(Jackle and Janning, 1998.8.10)

embryonic/larval midgut

(Jackle and Janning, 1998.8.10)

larval brain | presumptive

(Jackle and Janning, 1998.8.10)

stomodeum

(Jackle and Janning, 1998.8.10)

presumptive embryonic/larval central nervous system | restricted

(Jackle and Janning, 1998.8.10)

mesoderm

(Jackle and Janning, 1998.8.10)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

adult stage

adult central complex

(Vosshall, 1998.8.10)

adult brain | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, low level of expression in the central brain.

(Vosshall, 1998.8.10)

In embryo, expression in hindgut weak; higher levels in other tissues; segmentally repeated pattern of staining may be in the PNS.

(Jackle and Janning, 1998.8.10)

In third instar larva, expression in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(2)10521

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (0)

External Crossreferences and Linkouts ( 0 )

Comments

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P2374

(Meister and Braun, 1995.10)

P{PZ}l(2)10521¹⁰⁵²¹

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(2)10521¹⁰⁵²¹

(FlyBase, 1992-)

l(2)10521

(Gene Disruption Project members, 2015.10.8)

Name Synonyms

Secondary FlyBase IDs

FBti0000439

References (10)

Report Sections

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