FlyBase Insertion Report: Dmel\P{PZ}frc[00073]

General Information

Symbol

Dmel\P{PZ}frc⁰⁰⁰⁷³

Species

D. melanogaster

Name

FlyBase ID

FBti0005396

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)00073

Affected gene(s)

Coq4, Ecol\lacZ, frc

Viability / fertility

Associated allele(s)

Coq4⁰⁰⁰⁷³, Ecol\lacZ^frc-00073, frc⁰⁰⁰⁷³

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 74C1 )

Sequence Location

3L:17,433,113..17,433,113 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:17,433,113..17,433,113 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

74C1 (inferred by FlyBase from sequence location)

Cytological location (reported)

74C1-74C2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ026152

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Coq4

(FlyBase, 1996-)

Ecol\lacZ

(Goto et al., 2001, Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

frc

(Negreiros et al., 2018, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Coq4⁰⁰⁰⁷³

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^frc-00073

Phenotypic Class Phenotype Manifest in Detailed Description

frc⁰⁰⁰⁷³

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

larval brain

(Meister and Braun, 1995.10)

gonad

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

adult central complex | restricted

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, expressed in a discrete region of the central brain.

(Vosshall, 1998.8.10)

In wandering third instar larva, low level of β-gal expression in larval brain and gonad; higher levels in imaginal discs (which discs not specified).

(Meister and Braun, 1995.10)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)00073

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11484

P{PZ}frc⁰⁰⁰⁷³ Coq4⁰⁰⁰⁷³ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3L:17398372-17398373 confirmed by FlyBase alignment of dbGSS accession AQ026152 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

P{PZ}frc⁰⁰⁰⁷³

(FlyBase, 2005, FlyBase, 1992-)

P{PZ}l(3)00073⁰⁰⁰⁷³

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)00073⁰⁰⁰⁷³

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000459

References (16)

Report Sections

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