FlyBase Insertion Report: Dmel\P{PZ}blot[01658]

General Information

Symbol

Dmel\P{PZ}blot⁰¹⁶⁵⁸

Species

D. melanogaster

Name

FlyBase ID

FBti0005436

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)01658

Affected gene(s)

blot, Ecol\lacZ

Viability / fertility

Associated allele(s)

blot⁰¹⁶⁵⁸, Ecol\lacZ^blot-01658

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 74B1 )

Sequence Location

3L:17,421,200..17,421,200 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:17,421,200..17,421,200 [+]

Orientation

Cytological location (computed by FlyBase)

74B1 (inferred by FlyBase from sequence location)

Cytological location (reported)

74B1-74B2 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

G00742

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

blot

(BDGP Project Members, 1994-1999)

Ecol\lacZ

(Xi et al., 2003, Johnson et al., 1999, Ledent et al., 1998, Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

blot⁰¹⁶⁵⁸

Phenotypic Class Phenotype Manifest in Detailed Description

Ecol\lacZ^blot-01658

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 11

Malpighian tubule primordium

(Johnson et al., 1999)

embryonic stage 12 -- 13

Malpighian tubule tip cell primordium

(Johnson et al., 1999)

embryonic stage 14 -- 15

embryonic Malpighian tubule tip cell

(Johnson et al., 1999)

embryonic stage 16

cell | subset of larval ventral nerve cord | segmentally repeated

(Johnson et al., 1999)

third instar larval stage

embryonic/larval Malpighian tubule

(Johnson et al., 1999)

larval central nervous system | restricted

Comment: single cells on surface of brain lobes and ventral nerve cord

(Johnson et al., 1999)

ring gland

(Johnson et al., 1999)

eye-antennal disc

(Johnson et al., 1999)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

Malpighian tubule

(Meister and Braun, 1995.10)

adult stage

adult brain | restricted

(Vosshall, 1998.8.10)

lamina

(Vosshall, 1998.8.10)

adult central complex

(Vosshall, 1998.8.10)

Additional Information

Statement

Reference

In the adult, low level of expression in the central brain and lamina.

(Vosshall, 1998.8.10)

Marker for

Reflects expression of

blot

(Johnson et al., 1999)

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)01658

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11542

tap⁰¹⁶⁵⁸ P{PZ}blot⁰¹⁶⁵⁸ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{PZ}blot⁰¹⁶⁵⁸

(FlyBase, 1992-)

P{PZ}l(3)01658⁰¹⁶⁵⁸

(BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}tap⁰¹⁶⁵⁸

(FlyBase, 1992-)

P{ry^+t7.2=PZ}l(3)01658⁰¹⁶⁵⁸

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000499

References (11)

Report Sections

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