FlyBase Insertion Report: Dmel\P{PZ}scny[02331]

General Information

Symbol

Dmel\P{PZ}scny⁰²³³¹

Species

D. melanogaster

Name

FlyBase ID

FBti0005455

Feature type

transposable_element_insertion_site

Description

Inserted Element

P{PZ}

LINE ID

l(3)02331

Affected gene(s)

Ecol\lacZ, scny

Viability / fertility

Associated allele(s)

Ecol\lacZ⁰²³³¹, scny⁰²³³¹

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 64E7 )

Sequence Location

3L:5,770,681..5,770,681 [+]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{PZ}.BDGP

Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{PZ}; most lines have a lethal or sterile phenotype. The P{PZ} construct carries a ry[+] visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue.

(Spradling et al., 1999)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:5,770,681..5,770,681 [+]

(BDGP Project Members, 1994-1999)

Orientation

Cytological location (computed by FlyBase)

64E7 (inferred by FlyBase from sequence location)

Cytological location (reported)

64E8-64E12 (in situ hybridization reported)

(BDGP Project Members, 1994-1999)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AQ034051

(FlyBase, 1992-)

Inserted Element

Construct

P{PZ}

Size (Kb)

14.545

Component Allele(s)

l(3)87Df^7.2

ry^+t7.2

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Harvie et al., 1998, Russell et al., 1998, Vosshall, 1998.8.10, BDGP Project Members, 1994-1999)

scny

(Ribaya et al., 2009, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ⁰²³³¹

Phenotypic Class Phenotype Manifest in Detailed Description

scny⁰²³³¹

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

prothoracic gland

(Harvie et al., 1998)

wandering third instar larval stage

imaginal disc

(Meister and Braun, 1995.10)

embryonic/larval fat body

(Meister and Braun, 1995.10)

embryonic/larval optic lobe

(Harvie et al., 1998)

larval ventral nerve cord

(Harvie et al., 1998)

eye disc

(Harvie et al., 1998)

Additional Information

Statement

Reference

During the second larval instar-third larval instar, expressed in lateral secretory cells (prothoracic gland) of the ring gland. Expression in the larval CNS exhibits a punctate pattern.

(Harvie et al., 1998)

Marker for

Reflects expression of

Reporter construct used in assay

Data on Genetic Line

Line ID

l(3)02331

(Gene Disruption Project members, 2001-)

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

11565

P{PZ}scny⁰²³³¹ ry⁵⁰⁶/TM3, ry^RK Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Comments

Location 3L:5744628-5744629 confirmed by FlyBase alignment of dbGSS accession AQ034051 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

P{PZ}Usp36⁰²³³¹

P{PZ}l(3)02331⁰²³³¹

(FlyBase, 2005, BDGP Project Members, 1994-1999, FlyBase, 1992-)

P{PZ}scny⁰²³³¹

P{ry^+t7.2=PZ}l(3)02331⁰²³³¹

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

FBti0000515

References (14)

Report Sections

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