FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Insertion: Dmel\P{lacW}Pknk11209
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General Information
Symbol
Dmel\P{lacW}Pknk11209
Species
D. melanogaster
Name
FlyBase ID
FBti0006325
Feature type
transposable_element_insertion_site
Description
Inserted Element
P{lacW}
LINE ID

l(2)k11209

Affected gene(s)
Ecol\lacZ, Pkn
Viability / fertility
Associated allele(s)
Ecol\lacZPkn-k11209, Pknk11209
Stock availability
2 publicly available
Genomic Location
Chromosomal Location
2R ( 45C1 )
Sequence Location
2R:9,284,827..9,284,827 [-]
Target / Docking site
Genomic Maps
Member of Large Scale Dataset(s)
Dataset
TI_set_P{lacW}.BDGP
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

(Bellen et al., 2004)

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Spradling et al., 1999)
Detailed Mapping Data
Chromosome (arm)
Sequence Location
2R:9,284,827..9,284,827 [-]
(Sass and Ostrow, 2014)
Orientation

-

(Sass and Ostrow, 2014)
Cytological location (computed by FlyBase)
45C1 (inferred by FlyBase from sequence location)
Cytological location (reported)
45C3--45C4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
45C3-45C4 (in situ hybridization reported)
(BDGP Project Members, 1994-1999)
Insertion into Natural transposon
Comments concerning location

Insertion site reported as 2R:5172332 in release 5 coordinates with an 8 bp repeat of ATCTGAGC.

(Sass and Ostrow, 2014)

Originally reported at the equivalent of genome location 2R:9284828 (release 6 coordinates) and on the positive strand.

(BDGP Project Members, 1994-1999)
Sequence Data
Flanking sequence
AQ025955
(FlyBase, 1992-)
Inserted Element
Construct
P{lacW}
Size (Kb)

10.691

Component Allele(s)
w+mC
Molecular map
Affected Gene(s)
Insertion may affect gene
Ecol\lacZ
(BDGP Project Members, 1994-1999)
Pkn
(Sass and Ostrow, 2014, BDGP Project Members, 1994-1999)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID

l(2)k11209

(Gene Disruption Project members, 2001-)
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (2)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

Location 2R:4796641-4796642 confirmed by FlyBase alignment of dbGSS accession AQ025955 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b.

(FlyBase, 2005)

This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).

(FlyBase, 1992-)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
P[lacW]l(2)k11209
(Sass and Ostrow, 2014)
P{lacW}Pknk11209
(FlyBase, 2005, FlyBase, 1992-)
P{lacW}l(2)rG232k11209
(BDGP Project Members, 1994-1999, FlyBase, 1992-)
P{lacW}v(2)rG232k11209
(FlyBase, 1992-)
l(2)k11209
(Sass and Ostrow, 2014, FlyBase, 1992-)
Name Synonyms
Secondary FlyBase IDs
    References (9)