Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
The insertion site for P{lacW}embk06303 is reported to be within exon 4 of emb (current exon 5).
l(2)k06303
Despite the P{lacW}embk06303 insertion being within 200bp of the fy transcription start site, it does not significantly compromise fy expression; flies heterozygous for P{lacW}embk06303 and an amorphic fy allele have no detectable tissue polarity phenotype when cultured at the restrictive temperature.
The nsertion site was reported to map within within the fourth exon. The orientation of the insertion was not reported.
The orientation of the insertion was not reported.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).