Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.
A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or sterile phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Reported to be at the same location as but in the opposite orientation to P{PZ}Rpt105643; the insertion site is therefore at the other end of the 8bp repeat.
l(2)k11110
The P{lacW}Rpt1k11110 insertion is within Rpt1, which is located within a long intron of CG17985. Homozygosity for the P{lacW}Rpt1k11110 chromosome results in second instar larval lethality, which is not rescued by expression of Rpt1Ubi-p63E.PS. This suggests that the homozygous lethality of the chromosome is at least in part due to an effect on CG17985.
This insertion was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was not verified as such prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Allan Spradling (spradling@mail1.ciwemb.edu).