FlyBase Insertion Report: Dmel\P{GawB}103Y

General Information

Symbol

Dmel\P{GawB}103Y

Species

D. melanogaster

Name

FlyBase ID

FBti0007274

Feature type

transposable_element_insertion_site

Also Known As

103Y, 103Y-Gal4, P[Gal4]103Y

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^103Y

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

X ( 2D )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

2D (in situ hybridization reported)

(Tettamanti et al., 1997)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Pauls et al., 2010, Aso et al., 2009, Garcia-Lopez et al., 2008, Urizar et al., 2007, Joiner et al., 2006, Suh et al., 2004, Belgacem and Martin, 2002, Rosay et al., 2001, Gatti et al., 2000, Zars et al., 2000, Armstrong et al., 1998, Tettamanti et al., 1997)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^103Y

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

Kenyon cell | subset

(Tettamanti et al., 1997)

mushroom body | restricted

(Tettamanti et al., 1997)

mushroom body pedunculus | restricted

(Tettamanti et al., 1997)

third instar larval stage

brain | restricted

(Gatti et al., 2000)

larval ventral nerve cord | restricted

(Gatti et al., 2000)

pupal stage P7

mushroom body

(Armstrong et al., 1998)

adult mushroom body alpha-lobe

(Armstrong et al., 1998)

adult mushroom body beta-lobe

(Armstrong et al., 1998)

adult mushroom body gamma-lobe

(Armstrong et al., 1998)

adult mushroom body alpha'-lobe

(Armstrong et al., 1998)

adult stage

protocerebrum | restricted

(Gatti et al., 2000)

mushroom body calyx

(Rosay et al., 2001)

Kenyon cell | subset

(Tettamanti et al., 1997)

ellipsoid body | restricted

(Gatti et al., 2000)

neuron of protocerebrum | subset

(Gatti et al., 2000)

adult central complex | restricted

(Gatti et al., 2000)

fan-shaped body | restricted

(Gatti et al., 2000)

neuron | subset

(Gatti et al., 2000)

Kenyon cell

(Rosay et al., 2001)

lobe system of mushroom body | restricted

(Tettamanti et al., 1997)

adult mushroom body alpha-lobe

(Armstrong et al., 1998)

adult mushroom body beta-lobe

(Armstrong et al., 1998)

adult mushroom body

(Suh et al., 2004, Belgacem and Martin, 2002, Rosay et al., 2001, Gatti et al., 2000, Armstrong et al., 1998)

adult antennal lobe

(Gatti et al., 2000)

gamma Kenyon cell

(Pauls et al., 2010)

alpha/beta Kenyon cell

(Pauls et al., 2010)

alpha'/beta' Kenyon cell

(Pauls et al., 2010)

adult mushroom body gamma-lobe

(Pauls et al., 2010, Armstrong et al., 1998)

adult mushroom body alpha'-lobe

(Pauls et al., 2010, Armstrong et al., 1998)

mushroom body beta lobe core layer

(Aso et al., 2009)

mushroom body alpha lobe core layer

(Aso et al., 2009)

medulla

(Aso et al., 2009)

anterior optic tubercle

(Aso et al., 2009)

glial cell of adult brain | faint

(Aso et al., 2009)

adult mushroom body alpha-lobe

(Pauls et al., 2010)

adult mushroom body beta-lobe

(Pauls et al., 2010)

adult mushroom body beta'-lobe

(Pauls et al., 2010)

adult stage | male

adult mushroom body gamma-lobe

(Aso et al., 2009)

mushroom body beta lobe surface layer | intense

(Aso et al., 2009)

mushroom body alpha lobe surface layer | intense

(Aso et al., 2009)

mushroom body beta lobe posterior layer | intense

(Aso et al., 2009)

mushroom body alpha lobe posterior layer | intense

(Aso et al., 2009)

mushroom body beta' anterior-posterior layer | intense

(Aso et al., 2009)

mushroom body alpha' middle layer | faint

(Aso et al., 2009)

mushroom body beta' middle layer | faint

(Aso et al., 2009)

mushroom body alpha' anterior-posterior layer | intense

(Aso et al., 2009)

adult antennal lobe

(Aso et al., 2009)

fan-shaped body | faint

(Aso et al., 2009)

lobula

(Aso et al., 2009)

lobula plate

(Aso et al., 2009)

adult protocerebrum | restricted

(Aso et al., 2009)

inferior neuropils | faint

(Aso et al., 2009)

adult lateral horn | faint

(Aso et al., 2009)

adult antennal nerve | faint

(Aso et al., 2009)

adult deutocerebrum | faint

(Aso et al., 2009)

adult tritocerebrum | faint

(Aso et al., 2009)

adult gnathal ganglion | faint

(Aso et al., 2009)

adult pars intercerebralis | faint

(Aso et al., 2009)

Additional Information

Statement

Reference

Adult expression is observed in the mushroom body and antennal lobe, in a band in the fan-shaped body, in a ring around the ellipsoid body of the central body complex, and in scattered neurons in the protocerebrum. Third instar larval expression is seen in various parts of the brain and in the thoracic ganglia.

(Gatti et al., 2000)

Larval expression begins abruptly at hatching, and is observed in most of the mushroom body, except at the core of Kenyon cells clusters, and in the stalk of the mushroom body with 2-fold concentric organization.

(Tettamanti et al., 1997)

Marker for

Kenyon cell

(Rosay et al., 2001)

mushroom body

(Suh et al., 2004, Belgacem and Martin, 2002, Gatti et al., 2000)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Pauls et al., 2010, Aso et al., 2009)

Ecol\lacZ^UAS.cBa

(Armstrong et al., 1998)