FlyBase Insertion Report: Dmel\P{lacW}trn[S064117]

General Information

Symbol

Dmel\P{lacW}trn^S064117

Species

D. melanogaster

Name

FlyBase ID

FBti0009640

Feature type

transposable_element_insertion_site

Also Known As

trn-lacZ, tartan-lacZ

Description

Inserted Element

P{lacW}

LINE ID

Affected gene(s)

Ecol\lacZ, trn

Viability / fertility

Associated allele(s)

Ecol\lacZ^trn-S064117, trn^S064117

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 70A1 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{lacW}.S

Description

A set of third chromosome mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal or semi-lethal phenotype. The P{lacW} construct carries a w^+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

(Deak et al., 1997)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

70A1 (near gene of known cytology)

Cytological location (reported)

70A (in situ hybridization reported)

(Deak et al., 1997)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{lacW}

Size (Kb)

10.691

Component Allele(s)

w^+mC

Molecular map

Affected Gene(s)

Insertion may affect gene

Ecol\lacZ

(Becam et al., 2011, Mao et al., 2008, Adachi-Yamada et al., 2005, Milan et al., 2001, Salzberg et al., 1997)

trn

(Sharrock et al., 2022, Roy et al., 2014, Salzberg et al., 1997)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lacZ^trn-S064117

Phenotypic Class Phenotype Manifest in Detailed Description

trn^S064117

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc | restricted

(del Alamo Rodriguez et al., 2004)

eye disc morphogenetic furrow

(Mao et al., 2008)

eye disc posterior to the morphogenetic furrow

(Mao et al., 2008)

photoreceptor cell R1

(Mao et al., 2008)

photoreceptor cell R6

(Mao et al., 2008)

photoreceptor cell R7

(Mao et al., 2008)

Additional Information

Statement

Reference

EcollacZ^trn-S064117 drives expression in a dynamic pattern in the eye disc; initially at the morphogenetic furrow of the eye, at a lower level in all cells immediately posterior to the morphogenetic furrow, then becoming restricted to R1, R6, and &7 photoreceptor cells.

(Mao et al., 2008)

Larval expression in the dorsal mesothoracic disc is excluded from the center of the wing pouch, in a pattern complementary to that of sal. Representative image can be seen in FBrf074525, Figure 4 K.

(del Alamo Rodriguez et al., 2004)

Marker for

Reflects expression of

trn

(Mao et al., 2008, del Alamo Rodriguez et al., 2004, Milan et al., 2002, Salzberg et al., 1997)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

4550

y¹ w^67c23; P{lacW}trn^S064117/TM3, Sb¹ Ser¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

P{lacW}l(3)S064117^S064117

(FlyBase, 1992-)

P{lacW}trn^S064117

(FlyBase, 1992-)

tartan-lacZ

(Becam et al., 2011, Milan et al., 2002)

trn-Z

(Becam et al., 2011)

trn-lacZ

(Becam et al., 2011, Mao et al., 2008, del Alamo Rodriguez et al., 2004)

trn^s064117-lacZ

(del Alamo Rodriguez et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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