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General Information
Symbol
Dmel\P{lacW}Psf2SH0805
Species
D. melanogaster
Name
FlyBase ID
FBti0026079
Description
Inserted Element
Uses
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
LINE ID
Genomic Location
Chromosomal Location
2L ( 24E4 )
Sequence Location
2L:4,386,499..4,386,499
Target / Docking site
Member of Large Scale Dataset(s)
Description

Insertion lines from this collection were assessed for inclusion in the Gene Disruption Project collection.

A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).

Detailed Mapping Data
Chromosome (arm)
Sequence Location
2L:4,386,499..4,386,499
Orientation
Cytological location (computed by FlyBase)
24E4 (inferred by FlyBase from sequence location)
Cytological location (reported)
24F1 (reported as inferred from sequence location)
Insertion into Natural transposon
Comments concerning location

Insertion site previously reported at 2L:4386525..4386625 .

The insertion is reported to remove the last 64 amino acids of Psf2 and map to codon 151/215 of the Psf2 CDS. That maps to codon 149 of the FlyBase R5.56 Psf2 annotation, which uses a downstream translation start. The published coordinate ( 2L:4388852 ) from the same publication is in error as it maps to Tps1.

Sequence Data
Inserted Element
Construct
Location-dependent role
Size (Kb)

10.691

Associated alleles
Molecular map
Affected Gene(s)
Alleles and Phenotypes
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Related Aberration or Balancer
Aberration
Balancer
Stocks (1)
External Crossreferences and Linkouts ( 1 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
Comments
Comments

The orientation of the insertion was not reported.

Location 2L:4386524-4386626 confirmed by FlyBase alignment of dbGSS accession AB082282 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation confirmed.

Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{lacW}Psf2SH0805
P{lacW}l(2)SH0805SH0805
Name Synonyms
Secondary FlyBase IDs
    References (6)