A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
ScerGAL4NP3481 drives expression in around 10 uniglomerular antennal lobe projection neurons. Their cell bodies are positioned in the anterior-dorsal part of the antennal lobe and projections are detected to seven different glomeruli including VM7d. Labeling is also detected in one multiglomerular projection neuron. Its cell body is positioned ventrally and it innervates 14 different glomeruli including VC3l, VM6, VC5, VP1, VP4, VP2 and VP3.
Location X:20467695-20467696 refined by FlyBase alignment of dbGSS accession AG217159 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.