A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
sona-GAL4 is expressed in imaginal discs in later third instar larvae. In leg discs, staining occurs in the presumptive femur, tibia, and claw. In eye discs, staining is observed in the peripodial epithelium and in the photoreceptor clusters. Wing discs show a complex mosaic pattern.
Location 2R:19275185-19275186 refined by FlyBase alignment of dbGSS accession AG219087 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.