A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
Reporter expression is observed in late embryos in hemocytes, fat body, and anal pads in a pattern that closely mirrors Plod transcript and protein expression.
ScerGAL4Plod-NP5633 drives expression in hemocytes, fat bodies, and anal pads in late-stage embryos.
Location 3L:11179975-11179976 refined by FlyBase alignment of dbGSS accession AG218910 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.