FlyBase Insertion Report: Dmel\P{GawB}NP6561

General Information

Symbol

Dmel\P{GawB}NP6561

Species

D. melanogaster

Name

FlyBase ID

FBti0037041

Feature type

transposable_element_insertion_site

Also Known As

NP6561

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP6561

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 73E5 )

Sequence Location

3L:17,049,512..17,049,512 [-]

Target / Docking site

Genomic Maps

JBrowse

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

3L:17,049,512..17,049,512 [-]

(FlyBase, 2005)

Orientation

Cytological location (computed by FlyBase)

73E5 (inferred by FlyBase from sequence location)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

AG219015

(FlyBase, 1992-)

Inserted Element

Construct

P{GawB}

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Pauls et al., 2010, Xiong et al., 2010, Li et al., 2009, Tanaka et al., 2008, Liu et al., 2006)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP6561

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

Additional Information

Statement

Reference

Approximately 7 fan-shaped F1 somata are labelled with ScerGAL4^NP6561. Neurites from these cells converge into a bundle that runs dorsally and the posteriorly into the fan-shaped body.

(Li et al., 2009)

Marker for

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Li et al., 2009, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

for^UAS.cWa

(Li et al., 2009)

Syt1^UAS.EGFP

(Li et al., 2009)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

105258

y^* w^*; P{GawB}NP6561 / TM6, P{UAS-lacZ.UW23-1}UW23-1

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

AG219015

Comments

Location 3L:17014770-17014771 refined by FlyBase alignment of dbGSS accession AG219015 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.

(FlyBase, 2005)

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Gal4-NP6561

(Li et al., 2009)

NP6561

(Flybrain Neuron Database et al., 2018.6.28, Kottler et al., 2013, Pauls et al., 2010, Hong et al., 2008, Tanaka et al., 2008, Liu et al., 2006)

NP6561-GAL4

(Xiong et al., 2010)

P{GawB}NP6561

(FlyBase, 1992-)

P{GawB}NP6561-3-1

(FlyBase, 2005, FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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