PBac{RB} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{RB}. The PBac{RB} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a "splice trap," a splice acceptor site derived from a D. melanogaster gene. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
The insertion site location is not known precisely because of low quality sequence in the region of the vector-flank junction. The location is at an unknown distance (probably < 100 bp) to the left (centromere-proximal) of 3R:8651102 (release 6), which is either within the SLIRP2 gene or between the 5' ends of the SLIRP2 and Mkk4 genes.
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