A set of mutant stocks derived by insertional mutagenesis using the P-element construct P{lacW}; most lines have a lethal phenotype. The P{lacW} construct carries a w+mC mini-white visible marker, Ecol\lacZ enhancer trap sequences, and bacterial sequences that allow plasmid rescue (FBrf0049800).
Complementation, rescue and P-element excision analysis demonstrates that the homozygous lethality of the 'SH0113' chromosome, plus the grk protein mislocalization defects seen in homozygous 'SH0113' germ-line clones, are not due to the P{lacW}SH0113 insertion (which is inserted near the wake gene), but are instead due to a second-site mutation that affects the Syx1A gene (this mutation is represented by the Syx1ASH0113 allele).
Complementation, rescue and P-element excision analysis demonstrates that the homozygous lethality of the 'SH0113' chromosome, plus the grk protein mislocalization defects seen in homozygous 'SH0113' germ-line clones, are not due to the P{lacW}SH0113 insertion (which is inserted near the wake gene), but are instead due to a second-site mutation that affects the Syx1A gene (this mutation is represented by the Syx1ASH0113 allele).