Additional P{EPgy2} insertion lines were generated, mapped, and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{EPgy2}; created and vetted by the Gene Disruption Project (GDP). The P{EPgy2} construct carries two visible markers, the mini-white marker w+mC and the mini-yellow marker y+mDint2, and Scer\UAS binding sites for the Scer\GAL4 transcriptional regulator. The GAL4-UAS system allows regulated expression of genes proximate to the site of the insertion: genes properly oriented with respect to the Scer\UAS sequences can be conditionally expressed via transgene-derived Scer\GAL4 activity.
The two flanks derived from inverse PCR (DX977691 and DX977692) align to opposite stands at exactly the same nucleotide site. During the course of the transposon mutagenesis studies of the Gene Disruption Project, such cases of the inverse PCR flanks aligning to the same site on opposite strands have been observed for a small minority of other P-element, piggyBac and Minos insertions (because of the 8 bp target site duplication that P-elements create when they insert and the convention we use to adjust for this duplication, the insertion site locations listed in FlyBase for these are generally 7 bp apart for P-elements). We infer that these are complex insertion events, but do not understand the structure of the inserted element(s) or in which orientation the insertion occurred relative to the reference genome.
10.908
EY20997
LINECOMMENT:The 5prime and 3prime flanks map 7 nt apart on opposite strands