PBac{WH} insertion lines from Exelixis were remapped and assessed for inclusion in the Gene Disruption Project collection; flanking sequence data were submitted to GenBank.
A set of transgenic insertion stocks derived by TE mobilization using the Tni\piggyBac-based construct PBac{WH}. The PBac{WH} construct carries the w+mC mini-white marker, a single long (199-bp) Scer\FRT site, and a terminal Scer\UAS site for Scer\GAL4-driven misexpression of adjacent genes. The FRT site allows Scer\FLP-mediated recombination between other FRT-containing elements, and thus can be used to generate molecularly defined deletions.
Flanking sequences map 60 nucleotides apart on release 5, consistent with two insertions or an insertion and a deletion.
Location 2R:12150748-12150749 determined by FlyBase alignment of dbGSS accession CZ483963 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.
Location 2R:12150747-12150748 determined by FlyBase alignment of dbGSS accession CZ483962 to D. melanogaster arm Release_4 and heterochromatin Release_3.2b. Insertion orientation revised.