FlyBase Insertion Report: Dmel\P{GawB}NP2297

General Information

Symbol

Dmel\P{GawB}NP2297

Species

D. melanogaster

Name

FlyBase ID

FBti0130407

Feature type

transposable_element_insertion_site

Also Known As

NP2297

Description

Inserted Element

P{GawB}

LINE ID

Affected gene(s)

Scer\GAL4

Viability / fertility

Associated allele(s)

Scer\GAL4^NP2297

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

TI_set_P{GawB}.NP

Description

A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w^+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.

(Hayashi et al., 2002)

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

P{GawB}

(Tanaka et al., 2008)

Size (Kb)

11.279

Component Allele(s)

Scer\GAL4^wB

w^+mW.hs

Molecular map

Affected Gene(s)

Insertion may affect gene

Scer\GAL4

(Pauls et al., 2010, Tanaka et al., 2008)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Scer\GAL4^NP2297

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

mushroom body medial lobe arborizing neuron 1

(Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)

mushroom body gamma lobe slice 5

(Shuai et al., 2015, Tanaka et al., 2008)

mushroom body beta' lobe slice 2

(Shuai et al., 2015, Tanaka et al., 2008)

mushroom body beta' anterior-posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe surface layer

(Tanaka et al., 2008)

mushroom body beta lobe posterior layer

(Tanaka et al., 2008)

mushroom body beta lobe slice 2

(Shuai et al., 2015, Tanaka et al., 2008)

mushroom body medial lobe arborizing neuron 7

(Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)

mushroom body output neuron 22

(Flybrain Neuron Database et al., 2018.6.28, Shuai et al., 2015, Tanaka et al., 2008)

calyx of adult mushroom body

(Tanaka et al., 2008)

pedunculus of adult mushroom body

(Tanaka et al., 2008)

adult antennal lobe projection neuron DA2 lPN

(Tanaka et al., 2012)

adult uniglomerular antennal lobe projection neuron | subset

(Tanaka et al., 2012)

adult antennal lobe projection neuron VM4 adPN

(Tanaka et al., 2012)

adult multiglomerular antennal lobe projection neuron | subset

(Tanaka et al., 2012)

adult brain | restricted

(Shuai et al., 2015)

mushroom body extrinsic neuron

(Shuai et al., 2015)

mushroom body beta lobe slice 1

(Shuai et al., 2015)

Additional Information

Statement

Reference

ScerGAL4^NP2297 drives expression in mushroom body extrinsic neurons, including mushroom body medial lobe arborizing neuron 1, mushroom body medial lobe arborizing neuron 7 and mushroom body calyx-pedunculus arborizing neuron 1, that innervate mushroom body gamma lobe slice, mushroom body beta lobe slices 1 and 2, and mushroom body beta' lobe slice 2.

(Shuai et al., 2015)

ScerGAL4^NP2297 drives expression in 7 uniglomerular projection neurons. Around 2 cell bodies are located in the anterio-dorsal part of the antennal lobe around 5 cell bodies can be found in the lateral part. These neurons project to three glomeruli VC3m. Labeling is also detected in one cell body in the adult subesophageal zone. This neuron projects to glomeruli D, DA2, DL5, DM3, DM6, V, VA6, VC1, VM7v, DA1, DA3, DA4l, DA4m, DC1, DC3, VA1s, VA1v, DL2v, VL1 VC3l, VL2a, VP4, VP2, VP3 and DC4.

(Tanaka et al., 2012)

Arborisation within the calyx and pedunculus is due to the mushroom body calyx-pedunculus arborizing neuron 1.

(Tanaka et al., 2008)

Marker for

mushroom body medial lobe arborizing neuron 7

(Tanaka et al., 2008)

mushroom body output neuron 22

(Tanaka et al., 2008)

Reflects expression of

Reporter construct used in assay

Mmus\Cd8a^UAS.cLa.EGFP

(Tanaka et al., 2012, Tanaka et al., 2008)

Avic\GFP^S65T.UAS

(Tanaka et al., 2008)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Kyoto

112868

w^*; P{GawB}NP2297 / CyO

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

NP2297

(Flybrain Neuron Database et al., 2018.6.28, Bouzaiane et al., 2015, Shuai et al., 2015, Tanaka et al., 2012, Pauls et al., 2010, Tanaka et al., 2008)

P{GawB}NP2297

(FlyBase, 1992-)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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