A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GT1}. The P{GT1} construct is described as a dual-tagged gene trap (FBrf0132344):it carries (1) w+mGT1 mini-white marker, designed to confer a pigmented eye phenotype only if the construct is inserted within a host gene, and (2) a promoter-less Scer\GAL4 gene which is expressed if it is produced as a fusion with the mRNA produced by the host gene. The construct also carries a Tn\neoR selectable marker (FBrf0132344).
ScerGAL4Mctp-BG01140b drives expression in in accessory cells in olfactory sensory organs of the third antennal segment and maxillary palps.
3' RACE supports insertion into Mctp gene.
In situ hybridisation with a Scer\GAL4 probe shows a cytological location of 55F1--55F2 for this insertion. Rapid amplification of cDNA ends (3' RACE) analysis shows that the vector in the BG01140 line is spliced to the Mctp gene contradicting the BDGP prediction at 75F in the MYPT-75D gene.