A set of transgenic insertion stocks derived by TE mobilization using the P-element construct P{GawB}. The P{GawB} construct carries a w+mW.hs mini-white visible marker and Scer\GAL4 driver/enhancer trap sequences. The GAL4-UAS system is a binary system using Scer\GAL4, a yeast transcription activator protein gene, and Scer\UAS, the DNA binding site for the GAL4 protein. The P{GawB} construct acts as an enhancer trap: expression of the GAL4 is sensitive to enhancers in the genomic region of an insertion. When paired with a construct or insertion carrying a reporter gene downstream of Scer\UAS, the reporter gene is driven by and reflects the expression of the GAL4.
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ScerGAL4NP394 drives expression in two pairs of mirror-symmetric neurons in the larval brain that cross the midline, located in the anterior supraesophageal ganglion. Positive signals obtained by GFP reconstitution across synaptic partners (GRASP) are observed between the neurons labelled with ScerGAL4NP394 and pdf-positive neurons. The neurons labelled by ScerGAL4NP394 are also detected with ScerGAL4NP423 and ScerGAL4NP867.
