FlyBase Insertion Report: Dmel\Mi{Trojan-lexA:QFAD.2}Gad1[MI09277-TlexA:QFAD.2]

General Information

Symbol

Dmel\Mi{Trojan-lexA:QFAD.2}Gad1^{MI09277-TlexA:QFAD.2}

Species

D. melanogaster

Name

FlyBase ID

FBti0168170

Feature type

transposable_element_insertion_site

Also Known As

Gad1-LexA, Gad-LexA, GAD1-Trojan-LexA

Description

Inserted Element

Mi{Trojan-lexA:QFAD.2}

LINE ID

Affected gene(s)

Gad1

Viability / fertility

Associated allele(s)

Ecol\lexA^{Gad1-MI09277-TlexA:QFAD.2}, Gad1^{MI09277-TlexA:QFAD.2}

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3L ( 64A5 )

Sequence Location

Target / Docking site

Mi{MIC}Gad1^MI09277

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

64A5 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

Mi{Trojan-lexA:QFAD.2}

(Diao et al., 2015)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

Gad1

(Diao et al., 2015)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

Ecol\lexA^{Gad1-MI09277-TlexA:QFAD.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Gad1^{MI09277-TlexA:QFAD.2}

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

first instar larval stage

GABAergic neuron | subset of larval ventral nerve cord

(Babski et al., 2019)

adult stage

medulla intrinsic neuron Mi4

(Davis et al., 2020)

GABAergic neuron

(Cheong et al., 2024)

Additional Information

Statement

Reference

Drives expression in GABAergic neurons.

(FlyBase Curators, 2017)

Marker for

GABAergic neuron

(Cheong et al., 2024)

Reflects expression of

Gad1

(Cheong et al., 2024, Babski et al., 2019)

Reporter construct used in assay

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Replacement descendant of (target)

Mi{MIC}Gad1^MI09277

(Diao et al., 2015)

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

60324

w^*; Mi{Trojan-lexA:QFAD.2}Gad1^{MI09277-TlexA:QFAD.2}/TM6B, Tb¹

External Crossreferences and Linkouts ( 0 )

Comments

phiC31-mediated recombination has been used to replace the attP cassette of the Mi{MIC} element with the intron phase 2 "Trojan lexA:QFAD " cassette from the pBS-KS-attB2-SA(2)-T2A-lexA:QFAD-Hsp70 plasmid.

(Diao et al., 2015)

Synonyms and Secondary IDs (17)

Reported As

Symbol Synonym

GAD-LexA

(Chen et al., 2022)

GAD1-T2A-LexA

(Byers et al., 2021)

GAD1-Trojan-LexA

(Cheong et al., 2024)

GAD1-lexA

(Guo et al., 2022)

GAD1^MI09277 Trojan LexA::QFAD

(Sizemore et al., 2023)

GAD1^MI09277-LexA:QFAD

(Aranha et al., 2017)

GAD^Mi09277-LexA

(Byers et al., 2021)

Gad-LexA

(Arez et al., 2021)

Gad1(MI09277)-LexA:QFAD

(Oliveira-Ferreira et al., 2023)

Gad1-LexA

(Babski et al., 2019, Aranha et al., 2017)

Gad1-LexA Gad1-Trojan-LexA-QFAD

(Nakamizo-Dojo et al., 2023)

Gad1-LexA:QFAD2

(Wohl et al., 2023)

Gad1-Trojan-LexA

(Jonaitis et al., 2025)

Mi{Trojan-lexA::QFAD.2}Gad1^{MI09277-TlexA:QFAD.2}

(Jonaitis et al., 2025)

Mi{Trojan-lexA:QFAD.2}Gad1^{MI09277-TlexA:QFAD.2}

(Estacio-Gómez et al., 2020)

Trojan-GAD-T2A-LexA

(Zhu et al., 2024)

gad1-LexA

(Lacin et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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