FB2026_03 , released September 17, 2026
Insertion: Dmel\Mi{Sema1a.AE}Sema1aMI00031-AE
Open Close
General Information
Symbol
Dmel\Mi{Sema1a.AE}Sema1aMI00031-AE
Species
D. melanogaster
Name
FlyBase ID
FBti0201275
Feature type
transposable_element_insertion_site
Also Known As
sema-1a artificial exon
Description
Inserted Element
Mi{Sema1a.AE}
LINE ID
Affected gene(s)
Sema1a
Viability / fertility
Associated allele(s)
Ecol\lexASema1a-MI00031-AE, Sema1aMI00031-AE
Stock availability
None publicly available
Genomic Location
Chromosomal Location
2L ( 29E1-29E3 )
Sequence Location
Target / Docking site
Mi{MIC}Sema1aMI00031
Member of Large Scale Dataset(s)
Dataset
Description
Detailed Mapping Data
Chromosome (arm)
Sequence Location
Orientation
Cytological location (computed by FlyBase)
29E1-29E3 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Sequence Data
Flanking sequence
Inserted Element
Construct
Mi{Sema1a.AE}
(Pecot et al., 2013)
Size (Kb)
Component Allele(s)
Molecular map
Affected Gene(s)
Insertion may affect gene
Sema1a
(Hernandez-Fleming et al., 2017, Xie et al., 2017, Pecot et al., 2013)
Variant Molecular Consequences
Phenotypic Data
Expression Data
Reporter Expression
distribution deduced from reporter (Gal4 UAS)
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult ellipsoid body R-neuron | subset
(Xie et al., 2017)
Additional Information
Statement
Reference

EcollexASema1a-MI00031-AE drives expression in nearly all ring neuron Rs, except a subset of R2 and R4m neurons.

(Xie et al., 2017)
 
Marker for
Reflects expression of
Sema1a
(Xie et al., 2017)
Reporter construct used in assay
Data on Genetic Line
Line ID
Origin as a multiple insertion line
Progenitor(s) within the Genome
Replacement descendant of (target)
Mi{MIC}Sema1aMI00031
(Pecot et al., 2013)
Related Aberration or Balancer
Aberration
Balancer
Stocks (0)
External Crossreferences and Linkouts ( 0 )
Comments
Comments

The attP cassette of the Mi{MIC} element has been replaced with an artificial exon that contains: 1) splice acceptor sequence, 2) Sema1a coding sequence, 3) FRT-stop-FRT, 4) sequences encoding 3xTag:V5, 5) 2A peptide, 6) lexA::p65, and 7) 3' UTR of Sema1a. In the absence of FLP, a transcription termination sequence prevents expression of tagged Sema1a, but when FLP is expressed the stop sequence is excised, allowing a Tag:V5 epitope to be added to the endogenous Sema1a protein, along with co-transcription of lexA::p65 and Sema1a.

(Pecot et al., 2013)
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Mi{Sema1a.AE}Sema1aMI00031-AE
Sema-1a-FSF
(Xie et al., 2017)
Sema-1a-V5
(Pecot et al., 2013)
sema-1a Artificial exon
(Pecot et al., 2013)
sema-1a artificial exon
(Hernandez-Fleming et al., 2017)
Name Synonyms
Secondary FlyBase IDs
    References (3)