FB2026_03 , released September 17, 2026
Recombinant Construct: Mi{Sema1a.AE}
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General Information
Symbol
Mi{Sema1a.AE}
FlyBase ID
FBtp0130814
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Description

The attP cassette of the Mi{MIC} element has been replaced with an artificial exon that contains: 1) splice acceptor sequence, 2) Sema1a coding sequence, 3) FRT-stop-FRT, 4) sequences encoding 3xTag:V5, 5) 2A peptide, 6) lexA::p65, and 7) 3' UTR of Sema1a. In the absence of FLP, a transcription termination sequence prevents expression of tagged Sema1a, but when FLP is expressed the stop sequence is excised, allowing a Tag:V5 epitope to be added to the endogenous Sema1a protein, along with co-transcription of lexA::p65 and Sema1a.

(Pecot et al., 2013)
Construct components
Component
Use(s)
Encoded product / tool
lexA::p65
Tagged with
Tag:V5
Also carries
FRT
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
Mi{MIC}
(Pecot et al., 2013)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{Sema1a.AE}
(FlyBase, 1996-)
Secondary FlyBase IDs
    References (2)