Symbol
Dmel\Mi{CadN.AE}CadNMI00393-AE
Species
D. melanogaster
Name
FlyBase ID
FBti0201276
Affected gene(s)
Viability / fertility
Associated allele(s)
Stock availability
Chromosomal Location
2L
(
36D1-36D3
)
Sequence Location
Member of Large Scale Dataset(s)
Cytological location (computed by FlyBase)
36D1-36D3 (near gene of known cytology)
Cytological location (reported)
Insertion into Natural transposon
Comments concerning location
Insertion may affect gene
Variant Molecular Consequences
Associated Allele
Phenotypic Data
Reporter construct used in assay
Origin as a multiple insertion line
Progenitor(s) within the Genome
Replacement descendant of (target)
Related Aberration or Balancer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Symbol Synonym
Mi{CadN.AE}CadNMI00393-AE
The attP cassette of the Mi{MIC} element has been replaced with an artificial exon that contains: 1) splice acceptor sequence, 2) the last 4680bp of the CadN ORF (isoform RD), 3) FRT-stop-FRT, 4) sequences encoding 3xTag:V5, 5) 2A peptide, 6) lexA::p65, and 7) 3' UTR of CadN. In the absence of FLP, a transcription termination sequence prevents expression of tagged CadN, but when FLP is expressed the stop sequence is excised, allowing a Tag:V5 epitope to be added to the endogenous CadN protein, along with co-transcription of lexA::p65 and CadN.