FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: Mi{CadN.AE}
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General Information
Symbol
Mi{CadN.AE}
FlyBase ID
FBtp0130815
Feature type
transgenic_transposable_element
Component Allele(s)
Size
Expression Data
Associated insertion(s)
Molecular map
Description and Uses
Description

The attP cassette of the Mi{MIC} element has been replaced with an artificial exon that contains: 1) splice acceptor sequence, 2) the last 4680bp of the CadN ORF (isoform RD), 3) FRT-stop-FRT, 4) sequences encoding 3xTag:V5, 5) 2A peptide, 6) lexA::p65, and 7) 3' UTR of CadN. In the absence of FLP, a transcription termination sequence prevents expression of tagged CadN, but when FLP is expressed the stop sequence is excised, allowing a Tag:V5 epitope to be added to the endogenous CadN protein, along with co-transcription of lexA::p65 and CadN.

Construct components
Component
Use(s)
Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Component Allele
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Mi{CadN.AE}
Secondary FlyBase IDs
    References (2)