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General Information
Symbol
P{lwB}
FlyBase ID
FBtp0000157
Feature type
transgenic_transposon
Component Allele(s)
Size
12.486
Expression Data
lacZ reporter
Associated insertion(s)
Molecular map
Description and Uses
Location-dependent role
Description
CV term
Qualifiers and info
Reference
transposon
Construct components
Product class / Tool use(s)
Encoded product / tool
Marker(s)
Description

P{lwB} is an enhancer trap construct that contains at its 5' end the relatively weak P\T promoter fused upstream of sequence encoding the lacZ reporter enzyme (the lacZ sequence is fused in-frame to the second exon of the P\T coding sequence).

The lacZ reporter encoded by the P{lwB} construct is expected to be localized to the nucleus, because it is fused to the N-terminal 128 amino acid residues of P\T (which have been shown to be sufficient for nuclear localization).

Component allele Ecol\lacZP\T.A92a
Description

Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo.

Component allele w+mW.hs
Description

Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.

Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene.

Sequence Data
Sequence (FB)
Associated Sequence Data
DNA Sequence
Extent
Segments and Size
Total Size

12.486

Left end
Right end
 
Features
CV term
Qualifiers and info
Reference
bacterial
selection
chimeric
gene
chimeric
gene
enhancer
trap
partial
rescue
reporter
gene
Component Alleles
Molecular data

Construct: In frame translational fusion of P\T to Ecol\lacZ, in the second exon of the P element. The fusion includes the P element promoter. The P-element 5' end is from pUChsneo.

Allele
Reference(s)
Molecular data

Expression of a fragment of w genomic DNA with a 2.2kb deletion of 3' flanking sequences and a reduction in the size of the largest intron to 356bp is driven by a 388bp fragment of an Hsp70 gene.

Construct: A 'mini-white' gene constructed by deletion of the XbaI-HindII fragment from the long 5' intron. Carried by the W6 and W8 series of vectors.

Expression Data
Reporter Expression
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Information
Statement
Reference

Basal pattern of stripes, restricted to epidermal clusters of cells. Expression localized to nucleus.

Expression pattern dependent upon position of insertion in genome. Basal pattern observed in approximately 30% of transformants: in extended germ band embryos, weak expression in dorsal epidermis, stronger in alternate segments.

 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
Comments

construct_comment: One of a series of constructs containing the lacZ enhancer trap lacZP\T.A92a, differing in selectable markers.

Stocks (14)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Secondary FlyBase IDs
    References (14)