FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{ftz-lacC}
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General Information
Symbol
P{ftz-lacC}
FlyBase ID
FBtp0000176
Feature type
transgenic_transposable_element
Component Allele(s)
Ecol\lacZftz.PC
Also Known As
ftz-lacZ
Size
18.592
Expression Data
lacZ reporter
Associated insertion(s)
9 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
ry+t7.2
Component allele Ecol\lacZftz.PC
Transgenic product class
Component
Use(s)
Regulatory region(s)
ftz_lacC
Encoded product / tool
lacZ
Description

A full length ftz promoter fragment which extends from -6.1kb (relative to the translation start site) to the second codon is fused in frame to lacZ.

(Hiromi et al., 1985)

Approximately 6kb of ftz 5' flanking sequence, including the zebra, neurogenic and upstream elements drives lacZ expression.

(Yu and Pick, 1995)
Sequence Data
Sequence (FB)
FlyBase-compiled
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size

18.592

Left end

P5\'

(Hiromi et al., 1985)
Right end

P3\'

(Hiromi et al., 1985)
 
Segments
Num
Orientation
Symbol
1
+
Car1_P5'(P5',R1)
2
+
EcoRI_site
3
-
001066
4
-
ftz::lacZ_10.3(K,u)
5
+
001067
6
+
Car20_P3'(H3,P3')
Reference
(Hiromi et al., 1985)
Phenotypic Data
Expression Data
Reporter Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 13 -- 14
parasegment 4
(Tremml and Bienz, 1989)
parasegment 8
(Tremml and Bienz, 1989)
parasegment 12
(Tremml and Bienz, 1989)
distribution deduced from reporter
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 10 -- 11
mesoderm | pair rule expression pattern
(Hiromi and Gehring, 1987)
ectoderm | pair rule expression pattern
(Hiromi and Gehring, 1987)
embryonic stage 13 -- 14
parasegment 10
(Tremml and Bienz, 1989)
parasegment 2
(Tremml and Bienz, 1989)
parasegment 6
(Tremml and Bienz, 1989)
embryonic stage 15 -- 16
presumptive embryonic/larval central nervous system
(Hiromi and Gehring, 1987)
radioisotope in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 17
organism | pair rule expression pattern
(Hiromi et al., 1985)
embryonic stage 6 -- 17
organism | pair rule expression pattern
(Hiromi et al., 1985)
embryonic stage 11 -- 17
neuroblast | restricted
(Hiromi et al., 1985)
embryonic stage 13 -- 17
larval ventral nerve cord | segmentally repeated
(Hiromi et al., 1985)
epidermis | pair rule expression pattern
(Hiromi et al., 1985)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
(Hiromi et al., 1985)
first instar larval stage
(Hiromi et al., 1985)
Additional Information
Statement
Reference

ftz is expressed in alternating parasegments in the germ band retracted embryo between parasgements 2 and 12.

(Tremml and Bienz, 1989)

In germ-band extended embryos, strong expression in the mesoderm and ectoderm, in even parasegments. In later embryos, expression is observed in the CNS. Expression pattern characteristic of that observed for ftz.

(Hiromi and Gehring, 1987)

In embryos, peak accumulation is from 4.5-10.5 hours after egg-laying.

(Hiromi et al., 1985)

In gastrulating embryos, the first stripe is posterior to the cephalic furrow. Seven stripes observed; most posterior stripe thicker and occasionally resolves as two stripes. During mid-embryogenesis expression also observed in pairs of large cells; assumed to be neuroblasts. The most conspicuous of these pairs flank the ventral midline, one pair per segment. Observed stripes in epidermis correspond to parasegments, not segments. Expression throughout the ventral nerve cord (not in pair-rule pattern).

(Hiromi et al., 1985)
 
Marker for
Reflects expression of
ftz
(Franke and Baker, 1999, Doe, 1992, Tremml and Bienz, 1989, Hiromi and Gehring, 1987, Hiromi et al., 1985)
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{ftz/lacC}
(Hiromi et al., 1985)
descendant(s)
Comments

construct_comment: Defined 3 regulatory regions: the zebra element, the neurogenic element, and the upstream element.

(Hiromi and Gehring, 1987)

report comment: Contains the ftz "zebra element", the "neurogenic element" and the "upstream element" enhancers.

(Hiromi and Gehring, 1987)

construct_comment: One of a series of lacZ reporter constructs designed to characterize 5\' regulatory elements of ftz.

(Hiromi et al., 1985)
Stocks (62)
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (13)
References (56)