FlyBase Recombinant Construct Report: P{twi-βgal-1.4a}

General Information

Symbol

P{twi-βgal-1.4a}

FlyBase ID

FBtp0000678

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^twi.1.4a

Size

Expression Data

lacZ reporter

Associated insertion(s)

1 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mW.hs

Component allele Ecol\lacZ^twi.1.4a

Transgenic product class

Component

Use(s)

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Description

lacZ reporter expression driven by 1.4kb fragment of 5' twi regulatory sequences. Coding region is tagged with a nuclear localization signal and flanked at the 3' end with 3' Act5C sequences.

(Thisse et al., 1991)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^twi.1.4a

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

mesonotum & myoblast

(Sudarsan et al., 2001)

embryonic stage 4

mesoderm anlage

(Thisse et al., 1991)

organism | ventral

(Thisse et al., 1991)

gastrula stage

endoderm | anterior

(Thisse et al., 1991)

ventral furrow

(Thisse et al., 1991)

extended germ band stage

posterior midgut primordium

(Thisse et al., 1991)

anterior midgut primordium

(Thisse et al., 1991)

embryonic stage 9 -- 17

mesoderm

(Thisse et al., 1991)

embryonic stage 14

larval somatic muscle cell

(Thisse et al., 1991)

larval visceral muscle cell

(Thisse et al., 1991)

pupal stage

embryonic precursor cell of adult muscle

(Roy and VijayRaghavan, 1997)

Additional Information

Statement

Reference

This reporter reproduces the pattern of twi expression and was used to identify the myoblasts in the notum of the wing disc.

(Sudarsan et al., 2001, Thisse et al., 1991)

Marker for

Reflects expression of

twi

(Sudarsan et al., 2001, Thisse et al., 1991)

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{twi-βgal-1.4a}

(Thisse et al., 1991)

descendant(s)

Comments

construct_comment: One of a series of reporter constructs used to characterize twi 5\' regulatory sequences.

(Thisse et al., 1991)

Stocks (0)

Stocks containing insertion(s) of this recombinant construct

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

1.4kb twi-βgal

(Thisse et al., 1991)

P{twi-βgal-1.4a}

(FlyBase, 1996-)

twi-lacZ

(Sudarsan et al., 2001)

Secondary FlyBase IDs

References (7)

Report Sections

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