FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Recombinant Construct: P{PKA.mC.FRT}
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General Information
Symbol
P{PKA.mC.FRT}
FlyBase ID
FBtp0001141
Feature type
transgenic_transposable_element
Component Allele(s)
Mmus\PrkacamC.FRT.Act5C
Size
Expression Data
Associated insertion(s)
1 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
y+t7.7
Component allele Mmus\PrkacamC.FRT.Act5C
Transgenic product class
missense_variant
(Li et al., 1995)
Component
Use(s)
Regulatory region(s)
Act5C
Encoded product / tool
Mmus\Prkaca
Also carries
FRT
Description

An Act5C promoter is separated from sequences encoding a constitutively active form of Mmus\Prkaca ('mC*', contains the amino acid replacements H87Q and W196R) by an FRT cassette that contains the y+t7.7 marker.

(Li et al., 1995)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
pP{PKA.mC.FRT}
(Li et al., 1995)
descendant(s)
Comments

report comment: FRT-deleted (active) form of Act5C-PKA carried on descendant transposon P{PKA.mC.Act5C}.Once the FRT cassette is removed, the mouse PKA allele carried in this construct is constitutively active (reduced binding to regulatory subunit). PKA coding sequence terminates with human growth hormone polyA signal.

(Li et al., 1995)
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{PKA.mC.FRT}
(FlyBase, 1996-)
actin-FRT-mC*
(Li et al., 1995)
Secondary FlyBase IDs
    References (4)