The FRT cassette has been excised, juxtaposing Ubx promoter and enhancer sequences with Ubx and lacZ coding sequences.
Scer\FRT-Hsp70 cassette has been excised, juxtaposing Ubx promoter and enhancer sequences with Ubx and Ecol\lacZ coding sequences.
report comment: Ubx and lacZ coding sequences separated by multiple stop codons, then a Ubx IRES (internal ribosome entry site); appears to function as a dicistronic mRNA.
report comment: Ubx and lacZ coding sequences separated by multiple stop codons, then a Ubx IRES (internal ribosome entry site); appears to function as a dicistronic mRNA.