FlyBase Recombinant Construct Report: P{UAS-GFP::lacZ.nls}

UASt regulatory sequences drive expression of a fusion protein comprising the Tag:NLS(SV40-largeT) nuclear localization signal, the GFP coding region and the lacZ coding region (the lacZ coding region has been added with a view to stabilizing GFP expression in vivo).

(Shiga et al., 1996)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Avic\GFP^{UAS.lacZ,Tag:NLS(SV40-largeT)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAS-GFP::lacZ.nls}

(Shiga et al., 1996)

descendant(s)

Comments

construct_comment: Provides vital cell marker that can be targeted via GAL4/UAS binary system.

(Shiga et al., 1996)

report comment: Sequences for SV40 nuclear localization signal fused at 5\' end of GFP sequences.

(Shiga et al., 1996)

Stocks (6)

Stocks containing insertion(s) of this recombinant construct

Bloomington

6451

y¹ w^*; P{UAS-GFP::lacZ.nls}15.3

6452

y¹ w^*; P{UAS-GFP::lacZ.nls}30.1

6397

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

Kyoto

108781

P{UAS-GFP::lacZ.nls}15.2, y¹ w^*

108782

y¹ w^*; P{UAS-GFP::lacZ.nls}30.1

108768

w¹¹¹⁸; P{Hml-GAL4.G}6-4 P{UAS-GFP::lacZ.nls}15.1 P{UAS-GFP.S65T}Myo31DF^T2

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (28)

Reported As

Symbol Synonym

GFPN-lacZ

(FlyBase, 1996-)

P{UAS-GFP::lacZ.N}

(FlyBase, 1996-)

P{UAS-GFP::lacZ.nls}

(Liu et al., 2008, Bogwitz et al., 2005, FlyBase, 1996-)

UAS-GFP

(Dolezal et al., 2005)

UAS-GFP-nuclear lacZ

(Suster et al., 2003)

UAS-GFP::LacZ.nls

(Mao and Davis, 2009)

UAS-GFP::lacZ

(Lam et al., 2008)

UAS-GFP::lacZ.nls

(Manning et al., 2012, Barrett et al., 2008, Wong et al., 2008, Zheng et al., 2008, Taniguchi et al., 2007)

UAS-GFP:LacZ.nls

(Scantlebury et al., 2010)

UAS-GFP:NLS

(Manning et al., 2012)

UAS-GFPN-lacZ

(Dutta et al., 2005, Ribeiro et al., 2004, Sugimura et al., 2003)

UAS-GFPNLSlacZ

(Uv et al., 2000)

UAS-GFPNlacZ

(Wolf and Schuh, 2000)

UAS-LacZ::GFP.nls

(Freer et al., 2011)

UAS-NLS-GFP-lacZ

(Chen et al., 1998)

UAS-gfpn-lacZ

(Shiga et al., 1996)

UAS-gfpnlacZ

(Chihara and Hayashi, 2000, Ikeya and Hayashi, 1999)

UAS-lacZ-GFP

(Reich et al., 1999)

UAS-lacZnls

(Kondo and Hayashi, 2019)

UAS-nGFP

(Manning et al., 2012)

UAS-nGFP-LacZ

(Irisarri et al., 2009, Lavista-Llanos et al., 2002)

UAS-nlacZ::GFP

(Lee, 2013)

UAS-nls-GFP

(Wreden et al., 2017)

UAS-nlslacZ

(Fan et al., 2005)

UAS-nuc-GFP

(Freer et al., 2011)

UAS-nuclear GFP-lacZ

(Brand, 1999)

UAS.GFPNlacZ

(Takaesu et al., 2002)

pUAST-GFPnLacZ

(Fuse et al., 2003)

Secondary FlyBase IDs

References (37)

Report Sections

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