A pentamer of a 38bp region of the ninaE promoter (each copy of the 38bp sequence contains the minimal 29bp fragment protected by gl protein in a footprinting assay plus additional flanking nucleotides, with a mutation of ATTG to CAGT 3' to the gl binding site) is fused upstream of a minimal Hsp27 promoter followed by sequence encoding lacZ tagged with a nuclear localization signal.
The reporter construct is expressed in all cells posterior to the morphogenetic furrow.
Expressed in all cells posterior to morphogenetic furrow (not exclusively in photoreceptor cells).
construct_comment: One of a series of lacZ reporter constructs used to assay function of ninaE promoter regions that contain glass protein binding sites.