UASt regulatory sequences drive expression of mature SP (lacking the signal sequence) that is tagged at the N-terminal end with Tag:polyHis. The protein produced cannot be secreted due to the lack of signal sequence.
report comment: Acp70AUAS.6x.T:His6 encodes a cytoplasmic form of the protein.
construct_comment: Progenitors are those of the plasmid, pP{UAS-Acp70A.6His}.
report comment: Acp70AUAS.6x.T:His6 encodes a cytoplasmic form of the protein.
construct_comment: Progenitors are those of the plasmid, pP{UAS-Acp70A.6His}.