Construct: First 455 amino acids of tor, containing the signal peptide, extracellular domain with a single amino acid replacement and transmembrane peptide, are fused to amino acids 2-501 of pll, containing the intracellular domain.
Residues 1-455 of tor13D, including the extracellular and transmembrane domain, fused to residues 163-501 of pll.