Starting with plasmid pWHL, an 1311bp fragment of the βTub56D gene intron was cloned upstream of a truncated heat shock promoter in front of a lacZ reporter gene at a unique StuI site in the multiple cloning site.
EcollacZβTub56D.AS1 reporter gene expression is observed initially in stage 14 embryos. No difference in staining pattern is observed as compared to EcollacZβTub56D.β1WHI.